产品名称

英文名称：Recombinant Trypsin

同义词

重组修饰胰蛋白酶

产品性质

CAS编号：9002-07-7

EC号：232-650-8

酶学委员会编号：3.4.21.4

别名：重组修饰胰蛋白酶

规格或纯度：≥4500 units/mg,测序级

英文名称：Recombinant Trypsin

单位定义：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

来源：Expressed in E. Coli, methylation modified.

储存温度：-20°C储存

运输条件：超低温冰袋运输

产品介绍：胰蛋白酶是一种丝氨酸蛋白酶，酶解赖氨酸及精氨酸C末端形成的肽键。重组胰蛋白酶不含任何其他酶的活性，不含有且不需要TPCK抑制其他酶活性。胰蛋白酶容易自切产生自身自切片段影响质谱分析结果，甲基化修饰和突变保证其不自切，从而也去除了因自切而产生的假糜蛋白酶的活性。 R141080胰蛋白酶是一种丝氨酸蛋白酶，可于赖氨酸及精氨酸C末端剪切肽键。胰蛋白酶广泛用于各种生物技术过程中如蛋白质酶解生产多肽；各种组织的细胞分离；蛋白质的酶解、测序；重组胰岛素生产等等。重组猪胰蛋白酶是由重组大肠杆菌表达生产，氨基酸序列与猪胰腺来源的阳离子型胰蛋白酶完全一致。酶切特异性相同。分子量为24kD，最适pH为7-10。活性受丝氨酸蛋白酶抑制剂如PMSF等的抑制，金属离子螯合剂如EDTA等抑制酶活。无动物源性重组生产，无外源性的病毒污染，如猪流感病毒、猪细小病毒等。纯度高无其它杂酶污染；酶切反应无其它副反应。稳定冻干粉，易于储存和运输；批量生产，产品质量稳定。 UNIT DEFINITIONOne USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path). 重组胰蛋白酶具有与动物源性胰蛋白酶相同的酶学性质。可替代胰蛋白酶使用，且有很多优势，如无杂酶活性，高稳定性，不自切，高活性；在使用中，无非特异性酶切片段及自切片段出现。用于特异性的蛋白质酶解、蛋白测序、制作肽谱图、蛋白质组学研究、Z-D胶上的肽段的胰酶消化等。Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion. R141080Trypsin is a member of the serine protease family.Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.Animal origin freeThe use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animalderived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.StablilityA sterile recombinant trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.High purity1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

WGK Germany：1

RTECS：YN5075000

敏感性：对光线敏感；对湿度敏感

象形图：

信号词：Danger

危险声明：H315 Causes skin irritationH319 Causes serious eye irritationH335 May cause respiratory irritationH334 May cause allergy or asthma symptoms or breathing difficulties if inhaled

预防措施声明：P261,P342+P311

个人防护装备：dust mask type N95 (US), Eyeshields, Faceshields, Gloves

产品包装